| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hamm, Evan P (12)
|
MAUN-0 |
32.26 |
32.84 |
0.58 |
| 1 |
Brewer, Owen M (11)
|
MACVAC |
34.99 |
32.95 |
-2.04 |
| 1 |
Tsang, Jonathan M (11)
|
MADSC |
39.78 |
36.36 |
-3.42 |
| 2 |
Krebs, Noah J (11)
|
MACAA |
36.63 |
33.52 |
-3.11 |
| 2 |
Kulp, Jaxon W (12)
|
MANHAC |
38.87 |
36.72 |
-2.15 |
| 3 |
Stuhltrager, Brenden M (12)
|
MAFSSC |
33.83 |
34.04 |
0.21 |
| 3 |
Hasse, Peter E (11)
|
MANHAC |
38.26 |
37.18 |
-1.08 |
| 4 |
Colino, Jase A (11)
|
MAFSSC |
36.30 |
35.60 |
-0.70 |
| 4 |
Hancock, Lucas (11)
|
MALPAC |
40.14 |
37.24 |
-2.90 |
| 5 |
Wobber, Thomas A (11)
|
MACYA |
35.33 |
35.81 |
0.48 |
| 5 |
Hendershot, Carter R (11)
|
MACYA |
39.14 |
37.35 |
-1.79 |
| 6 |
Mertz, Caleb J (12)
|
MADSC |
36.99 |
35.88 |
-1.11 |
| 6 |
Bucks, Christian M (11)
|
MACYA |
40.10 |
38.62 |
-1.48 |
| 7 |
Simes, Colby A (12)
|
MANHAC |
36.70 |
36.29 |
-0.41 |
| 7 |
Yarnell, William P (11)
|
MAACWS |
44.92 |
38.82 |
-6.10 |
| 8 |
Shaud, Harrison T (11)
|
MAEAAC |
36.19 |
36.47 |
0.28 |
| 8 |
Ludwig, Connor S (11)
|
MANHAC |
40.96 |
38.87 |
-2.09 |
| 9 |
Teets, Grayson C (11)
|
MAPACS |
39.99 |
39.89 |
-0.10 |
| 10 |
Buckwalter, Aidan J (12)
|
MAFSSC |
44.28 |
40.63 |
-3.65 |
| 11 |
Burrowes, Owen C (12)
|
MAFSSC |
39.99 |
41.29 |
1.30 |
| 12 |
Barcikowski, Caden M (11)
|
MDACSS |
41.69 |
41.45 |
-0.24 |
| 13 |
Sanderson, Gary M (11)
|
MAMARA |
38.69 |
42.31 |
3.62 |
| 14 |
Eckert, Jevin (11)
|
MACYA |
48.45 |
42.53 |
-5.92 |
| 15 |
Fetters, Aiden R (11)
|
MACYA |
53.53 |
43.45 |
-10.08 |
| 16 |
Byrnes, Dolan D (12)
|
MANHAC |
|
44.53 |
- |
| 17 |
Zimmerman, Kaleb M (11)
|
MANHAC |
44.93 |
45.51 |
0.58 |
| 18 |
Smith, Jacob W (12)
|
MADSC |
45.21 |
47.09 |
1.88 |
| 19 |
Slusser, Daanen M (11)
|
MAPACS |
50.32 |
47.28 |
-3.04 |
| 20 |
Walker, Evan J (11)
|
MANHAC |
48.37 |
49.70 |
1.33 |